Descriptions
Figure1. Expression of three mammailian proteins using the pcDNA3.3-TOPO vector in suspention-adapted Freestyle CHO-S or Freestyle 293-F cells were transfected in 30ml volumes with pc DNA 3.3-TOPO vectors and pcDNA3.1/V5-His TOPO vectors containing the relevant genes. Culture medium was sampled 4-6 days post transfection. EPO(A) and FIX(B) were quantified by ELISA. and lgG was quantified using immovbilized go at anti-human lgG(c). Each bar is the average of two experiments.
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Size
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pcDNA3.3-TOPO TA cloning kit
20 rxns
k8300-01
Native Proteins in Mamalian Cells
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