Cytopatch Instrument

Software integrates up to 20 modules Patch clamp: "Gold standard" for Ion Channel Screening Quality criteria for accurate shole-cell recordings: high seal resistance (Rseal>1GOhm)Rseal = 2.7GOhm(n=76, Kv1.5) high and stable membrane resistance (Rmem>500MOhm)94% stable whole-cell after gigaseal (Kv1.5), continuously monitored low access resistance (Ra<10MOhmRa=9.92MOhm(n=47, HEK hERG cells) stable whole-cell configuration for at least 15 min CytoPatchTM Chip Cytocentering channel to position a single cell one cell is positioned on top of the patch pipette by applying an accurate pressure controle The CytoPatchTM Pipette resembles a "real" patch pipette: glass surface for real gigaseals shape and diameter (ca. 2µm) no contamination of the tip of CytoPatchTM pipette: positive pressure until sealing Rpip ca. 8MOhm (Ver.1) or ca. 4MOhm (Ver.2) Advantage : high data quality due to stable gigaseals robust whole-cell recordings ? high success rates and high through-put Fig2. Micromachined patch-clapm chip with embedded microfluidie channels. A) Schematic cross section. B) Cross section of the contact area obtained by focused ion beam (FIB) and SEM technique. C) SEM image of the pipette structure. Fig.3 Contacting of cell and chip. The chip is sealed in a silicone rubber packaging that contains micro channels. These are contacted and filled via penetracting cannulaes, that are also used for cell and compound injection into the transport channel, pressure application for cell positioning and electrical contacting. CytoPatchTM Rapid Perfusion System Quality and flexibility: rapid perfusion system Continuous perfusion with extra-cellular solution Continous perfusion with compound, fast and precisely triggered Continuous pre-incubation with compound (e.g. antogonist) and subsequent second application (e.g. agonist) CytoPatchTM Rrapid Perfusion System Kinetics of hERG channel blockers Fluvoxamine and Astemizole Application of 10µM Fluvoxamine and 20nM Astemizole