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Preparation Instant Cells :
Instant Cells are stored in caps
Remove cap from liquid nitrogen container
Screw cap on a tube containing extracellular buffer
Shake tube for 2 minutes
Spin down the Instant Cells for 4 minutes
Resuspend the pellet in extracellular buffer
Use Instant Cells immediately for the assay or transfer them into
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Software integrates up to 20 modules
Patch clamp: "Gold standard" for Ion Channel Screening
Quality criteria for accurate shole-cell recordings:
high seal resistance (Rseal>1GOhm)Rseal = 2.7GOhm(n=76, Kv1.5)
high and stable membrane resistance (Rmem>500MOhm)94% stable whole-cell after gigaseal (Kv1.5), continuously monitored
-
Preparation Instant Cells :
Instant Cells are stored in caps
Remove cap from liquid nitrogen container
Screw cap on a tube containing extracellular buffer
Shake tube for 2 minutes
Spin down the Instant Cells for 4 minutes
Resuspend the pellet in extracellular buffer
Use Instant Cells immediately for the assay or transfer them into
-
Software integrates up to 20 modules
Patch clamp: "Gold standard" for Ion Channel Screening
Quality criteria for accurate shole-cell recordings:
high seal resistance (Rseal>1GOhm)Rseal = 2.7GOhm(n=76, Kv1.5)
high and stable membrane resistance (Rmem>500MOhm)94% stable whole-cell after gigaseal (Kv1.5), continuously monitored