labopass

Fig.1 Consistent preparation. Plasmid DNA was isolated from overnight cultures of pBluescript(SK+) transformed JM109 cells using LaboPass™ mini kit. Each lane represents 10ul of supercoiled, purified plasmid DNA out of 50ul of eluates. Lane M represent DNA standard. Fig.2 Enzyme digestion. Several kinds of plasmid DNA purified with LaboPass™ mini kit was digested with endonucleases.Lane M : Lambda-BstPI markerLane 2 : pUC18, host INVaF'Lane 3 : pUC18, host INVaF', digested with XbaILane 4 : pQE30, host JM109Lane 5 : pQE30, host JM109, digested with SmaILane 6 : pBluescript II SK(+), host XL1 blueLane 7 : pBluescript II SK(+), host XL1 blue, digested with XbaI Fig.3 pUC18 plasmid vector was isolated from 3 ml of overnight cultures(INV@) using the preparation kit of Cosmo's LaboPass™ mini kit (lane 1,2), company A(lane 3,4) and company B(lane 5,6). Elution was carried out with 50ul of ddH2O, and each 5ul from eluates was resolved on 1.0% agarose gel. M: lambda-HindIII DNA standard. Fig.4   Auto sequencing analysis. Plasmid DNA purified with LaboPass™ mini kit was subjected to auto sequencing on an ABI Prism™, model 377, version 3.3 sequencer.