Fig.1 Consistent preparation. Plasmid DNA was isolated from overnight cultures of pBluescript(SK+) transformed JM109 cells using LaboPass™ mini kit. Each lane represents 10ul of supercoiled, purified plasmid DNA out of 50ul of eluates. Lane M represent DNA standard.
Fig.2
Enzyme digestion. Several kinds of plasmid DNA purified with LaboPass™ mini kit was digested with endonucleases.Lane M : Lambda-BstPI markerLane 2 : pUC18, host INVaF'Lane 3 : pUC18, host INVaF', digested with XbaILane 4 : pQE30, host JM109Lane 5 : pQE30, host JM109, digested with SmaILane 6 : pBluescript II...